Ge dige manual

Phosphorylase B was labeled with CyDye DIGE fluor Cy3 minimal dye and separated using the Amersham WB electrophoresis gel. The gel was imaged with Amersham Typhoon using normal scan speed. The detection limit was 22 pg and the linear dynamic range (DR) was orders of magnitude. Cy™ 5 Cy3 log (pg phosphorylase B) log (pg phosphorylase B) Detect. 10 Ettan DIGE System User Manual Edition AB Analytical workflow The analytical workflow is used to find proteins of interest in an experiment. It includes defining the experimental design, preparing the samples, performing 2D separation of the proteins, scanning the ge ls and analyzing the gel images to find proteins of interest. 5. Finally, the gels are scanned using an Ettan DIGE Imager (GE Healthcare) to image each gel to detect the Cy2-, Cy3- and Cy5-labelled proteins. The image files are transferred to Progenesis SameSpots, Version (Nonlinear Dynamics) for gel alignment, spot detection and statistical analysis.

DIGE Gel and DIGE Buffer Kit User Manual Edition AB 8 Instructions for use 3 buffer from the buffer seal. Fig Add the anode buffer. Note: Avoid pouring the DIGE anode buffer onto the tubing by spreading the tubing elements apart using one hand while pouring the solution with the other hand. 3 Switch the separation unit on. 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE) technology is covered by US patent numbers 6,,, 6,, and 6,, and equivalent patents and patent applications in other countries and exclusively licensed from Carnegie Mellon University. GE Appliance - Download a Copy of Your Owner's Manual or Installation Instructions. You can find your appliance's installation instructions and/or learn about the use.

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